LAB SAFETY SUPPLY
Frequently asked questions
Common questions about LAB SAFETY SUPPLY from 3G Electric.
What pore sizes are available in LAB SAFETY SUPPLY bottle top filters?
LAB SAFETY SUPPLY bottle top filters are offered with two pore sizes: 0.22 µm and 0.45 µm. The 0.22 µm pore size is typically used for sterilization of aqueous solutions, as it retains microorganisms. The 0.45 µm pore size is used for clarification and removal of larger particles. Both pore sizes are available across various membrane diameters and upper capacities. Selection depends on the application: 0.22 µm for sterile filtration and 0.45 µm for general particulate removal. The PES membrane provides low protein binding and is suitable for biological solutions.
What membrane diameters and upper capacities are available for LAB SAFETY SUPPLY bottle top filters?
LAB SAFETY SUPPLY bottle top filters come in membrane diameters of 50 mm, 75 mm, and 90 mm. Upper capacities include 150 mL, 250 mL, 500 mL, and 1 L. The diameter and capacity can be matched to the volume being filtered and the desired flow rate. Larger diameters generally provide faster filtration for larger volumes. For example, a 90 mm diameter with 1 L capacity is suitable for filtering larger batches, while a 50 mm diameter with 150 mL capacity is used for smaller volumes. All combinations are sterile and use PES membranes.
Do LAB SAFETY SUPPLY bottle top filters include a stopper?
Some models of LAB SAFETY SUPPLY bottle top filters include a stopper, while others do not. The stopper allows the filter to be attached directly to a media bottle, creating a sealed connection for vacuum filtration. Models with a stopper are identified in the product specifications. If a stopper is not included, the filter can be used with a separate vacuum filtration setup. The choice depends on the user's preference and existing equipment. Both configurations are sterile and use PES membranes.
What is the membrane material used in LAB SAFETY SUPPLY bottle top filters?
LAB SAFETY SUPPLY bottle top filters use polyethersulfone (PES) membranes. PES is chosen for its low protein binding, which minimizes sample loss, and its compatibility with aqueous solutions. It also provides high flow rates and mechanical strength. The membrane is housed in a polypropylene body. PES membranes are suitable for filtering media, buffers, and biological solutions. They are resistant to a range of pH levels and can be used with common laboratory reagents. The filters are gamma sterilized and intended for single use.
Are LAB SAFETY SUPPLY bottle top filters sterile?
Yes, all LAB SAFETY SUPPLY bottle top filters are sterile. They are sterilized by gamma irradiation and individually packaged to maintain sterility until use. This makes them suitable for applications requiring aseptic conditions, such as media preparation and sterilization of biological solutions. The sterile packaging ensures that the filter is ready for immediate use in a laminar flow hood or clean bench. Users should follow standard aseptic techniques when opening the packaging and installing the filter. The filters are intended for single use only.
What applications are LAB SAFETY SUPPLY bottle top filters used for?
LAB SAFETY SUPPLY bottle top filters are used for vacuum filtration of aqueous solutions in laboratory settings. Common applications include sterilization of cell culture media, buffers, and biological solutions. The 0.22 µm pore size is used for sterilization, while 0.45 µm is used for clarification. They are also used in environmental testing, food and beverage analysis, and pharmaceutical research. The filters can be used with standard vacuum filtration manifolds. The choice of diameter and capacity depends on the volume to be filtered and the desired flow rate.
